Two novel dromedary camel bocaparvoviruses from dromedaries in the Middle East with unique genomic features.
Identifieur interne : 000B35 ( Main/Exploration ); précédent : 000B34; suivant : 000B36Two novel dromedary camel bocaparvoviruses from dromedaries in the Middle East with unique genomic features.
Auteurs : Patrick C Y. Woo ; Susanna K P. Lau ; Hoi-Wah Tsoi ; Nissy Georgy Patteril ; Hazel C. Yeung ; Sunitha Joseph ; Emily Y M. Wong ; Rubeena Muhammed ; Franklin W N. Chow ; Ulrich Wernery ; Kwok-Yung YuenSource :
- The Journal of general virology [ 1465-2099 ] ; 2017.
Descripteurs français
- KwdFr :
- Analyse de regroupements, Analyse de séquence d'ADN, Animaux, Bocavirus (), Bocavirus (génétique), Bocavirus (isolement et purification), Chameaux (virologie), Fèces (virologie), Génome viral, Infections à Parvoviridae (médecine vétérinaire), Infections à Parvoviridae (virologie), Ordre des gènes, Phylogénie, Réaction de polymérisation en chaîne, Similitude de séquences d'acides aminés, Émirats arabes unis.
- MESH :
- génétique : Bocavirus.
- isolement et purification : Bocavirus.
- médecine vétérinaire : Infections à Parvoviridae.
- virologie : Chameaux, Fèces, Infections à Parvoviridae.
- Analyse de regroupements, Analyse de séquence d'ADN, Animaux, Bocavirus, Génome viral, Ordre des gènes, Phylogénie, Réaction de polymérisation en chaîne, Similitude de séquences d'acides aminés, Émirats arabes unis.
English descriptors
- KwdEn :
- Animals, Bocavirus (classification), Bocavirus (genetics), Bocavirus (isolation & purification), Camelus (virology), Cluster Analysis, Feces (virology), Gene Order, Genome, Viral, Parvoviridae Infections (veterinary), Parvoviridae Infections (virology), Phylogeny, Polymerase Chain Reaction, Sequence Analysis, DNA, Sequence Homology, Amino Acid, United Arab Emirates.
- MESH :
- classification : Bocavirus.
- genetics : Bocavirus.
- isolation & purification : Bocavirus.
- veterinary : Parvoviridae Infections.
- virology : Camelus, Feces, Parvoviridae Infections.
- Animals, Cluster Analysis, Gene Order, Genome, Viral, Phylogeny, Polymerase Chain Reaction, Sequence Analysis, DNA, Sequence Homology, Amino Acid, United Arab Emirates.
Abstract
The recent emergence of Middle East respiratory syndrome (MERS) coronavirus and its discovery from dromedary camels has boosted interest in the search for novel viruses in dromedaries. While bocaparvoviruses are known to infect various animals, it was not known that they exist in dromedaries. In this study, we describe the discovery of two novel dromedary camel bocaparvoviruses (DBoVs), DBoV1 and DBoV2, from dromedary faecal samples in Dubai. Among 667 adult dromedaries and 72 dromedary calves, 13.9 % of adult dromedaries and 33.3 % of dromedary calves were positive for DBoV1, while 7.0 % of adult dromedaries and 25.0 % of dromedary calves were positive for DBoV2, as determined by PCR. Sequencing of 21 DBoV1 and 18 DBoV2 genomes and phylogenetic analysis showed that DBoV1 and DBoV2 formed two distinct clusters, with only 32.6-36.3 % amino acid identities between the DBoV1 and DBoV2 strains. Quasispecies were detected in both DBoVs. The amino acid sequences of the NS1 proteins of all the DBoV1 and DBoV2 strains showed <85 % identity to those of all the other bocaparvoviruses, indicating that DBoV1 and DBoV2 are two bocaparvovirus species according to the ICTV criteria. Although the typical genome structure of NS1-NP1-VP1/VP2 was observed in DBoV1 and DBoV2, no phospholipase A2 motif and associated calcium binding site were observed in the predicted VP1 sequences for any of the 18 sequenced DBoV2, and no start codons were found for their VP1. For all 18 DBoV2 genomes, an AT-rich region of variable length and composition was present downstream to NP1. Further studies will be crucial to understand the pathogenic potential of DBoVs in this unique group of animals.
DOI: 10.1099/jgv.0.000775
PubMed: 28613145
Affiliations:
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Le document en format XML
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Woo</name><affiliation><nlm:affiliation>Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong SAR.</nlm:affiliation><wicri:noCountry code="subField">Hong Kong SAR</wicri:noCountry></affiliation></author><author><name sortKey="Lau, Susanna K P" sort="Lau, Susanna K P" uniqKey="Lau S" first="Susanna K P" last="Lau">Susanna K P. Lau</name><affiliation><nlm:affiliation>Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The University of Hong Kong, Hong Kong SAR.</nlm:affiliation><wicri:noCountry code="subField">Hong Kong SAR</wicri:noCountry></affiliation></author><author><name sortKey="Tsoi, Hoi Wah" sort="Tsoi, Hoi Wah" uniqKey="Tsoi H" first="Hoi-Wah" last="Tsoi">Hoi-Wah Tsoi</name><affiliation><nlm:affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong SAR.</nlm:affiliation><wicri:noCountry code="subField">Hong Kong SAR</wicri:noCountry></affiliation></author><author><name sortKey="Patteril, Nissy Georgy" sort="Patteril, Nissy Georgy" uniqKey="Patteril N" first="Nissy Georgy" last="Patteril">Nissy Georgy Patteril</name><affiliation><nlm:affiliation>Central Veterinary Research Laboratory, Dubai, UAE.</nlm:affiliation><wicri:noCountry code="subField">UAE</wicri:noCountry></affiliation></author><author><name sortKey="Yeung, Hazel C" sort="Yeung, Hazel C" uniqKey="Yeung H" first="Hazel C" last="Yeung">Hazel C. Yeung</name><affiliation><nlm:affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong SAR.</nlm:affiliation><wicri:noCountry code="subField">Hong Kong SAR</wicri:noCountry></affiliation></author><author><name sortKey="Joseph, Sunitha" sort="Joseph, Sunitha" uniqKey="Joseph S" first="Sunitha" last="Joseph">Sunitha Joseph</name><affiliation><nlm:affiliation>Central Veterinary Research Laboratory, Dubai, UAE.</nlm:affiliation><wicri:noCountry code="subField">UAE</wicri:noCountry></affiliation></author><author><name sortKey="Wong, Emily Y M" sort="Wong, Emily Y M" uniqKey="Wong E" first="Emily Y M" last="Wong">Emily Y M. Wong</name><affiliation><nlm:affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong SAR.</nlm:affiliation><wicri:noCountry code="subField">Hong Kong SAR</wicri:noCountry></affiliation></author><author><name sortKey="Muhammed, Rubeena" sort="Muhammed, Rubeena" uniqKey="Muhammed R" first="Rubeena" last="Muhammed">Rubeena Muhammed</name><affiliation><nlm:affiliation>Central Veterinary Research Laboratory, Dubai, UAE.</nlm:affiliation><wicri:noCountry code="subField">UAE</wicri:noCountry></affiliation></author><author><name sortKey="Chow, Franklin W N" sort="Chow, Franklin W N" uniqKey="Chow F" first="Franklin W N" last="Chow">Franklin W N. Chow</name><affiliation><nlm:affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong SAR.</nlm:affiliation><wicri:noCountry code="subField">Hong Kong SAR</wicri:noCountry></affiliation></author><author><name sortKey="Wernery, Ulrich" sort="Wernery, Ulrich" uniqKey="Wernery U" first="Ulrich" last="Wernery">Ulrich Wernery</name><affiliation><nlm:affiliation>Central Veterinary Research Laboratory, Dubai, UAE.</nlm:affiliation><wicri:noCountry code="subField">UAE</wicri:noCountry></affiliation></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><affiliation><nlm:affiliation>Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong SAR.</nlm:affiliation><wicri:noCountry code="subField">Hong Kong SAR</wicri:noCountry></affiliation></author></analytic><series><title level="j">The Journal of general virology</title><idno type="eISSN">1465-2099</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Bocavirus (classification)</term><term>Bocavirus (genetics)</term><term>Bocavirus (isolation & purification)</term><term>Camelus (virology)</term><term>Cluster Analysis</term><term>Feces (virology)</term><term>Gene Order</term><term>Genome, Viral</term><term>Parvoviridae Infections (veterinary)</term><term>Parvoviridae Infections (virology)</term><term>Phylogeny</term><term>Polymerase Chain Reaction</term><term>Sequence Analysis, DNA</term><term>Sequence Homology, Amino Acid</term><term>United Arab Emirates</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de regroupements</term><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Bocavirus ()</term><term>Bocavirus (génétique)</term><term>Bocavirus (isolement et purification)</term><term>Chameaux (virologie)</term><term>Fèces (virologie)</term><term>Génome viral</term><term>Infections à Parvoviridae (médecine vétérinaire)</term><term>Infections à Parvoviridae (virologie)</term><term>Ordre des gènes</term><term>Phylogénie</term><term>Réaction de polymérisation en chaîne</term><term>Similitude de séquences d'acides aminés</term><term>Émirats arabes unis</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Bocavirus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Bocavirus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Bocavirus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Bocavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Bocavirus</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Infections à Parvoviridae</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Parvoviridae Infections</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Chameaux</term><term>Fèces</term><term>Infections à Parvoviridae</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Camelus</term><term>Feces</term><term>Parvoviridae Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cluster Analysis</term><term>Gene Order</term><term>Genome, Viral</term><term>Phylogeny</term><term>Polymerase Chain Reaction</term><term>Sequence Analysis, DNA</term><term>Sequence Homology, Amino Acid</term><term>United Arab Emirates</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de regroupements</term><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Bocavirus</term><term>Génome viral</term><term>Ordre des gènes</term><term>Phylogénie</term><term>Réaction de polymérisation en chaîne</term><term>Similitude de séquences d'acides aminés</term><term>Émirats arabes unis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The recent emergence of Middle East respiratory syndrome (MERS) coronavirus and its discovery from dromedary camels has boosted interest in the search for novel viruses in dromedaries. While bocaparvoviruses are known to infect various animals, it was not known that they exist in dromedaries. In this study, we describe the discovery of two novel dromedary camel bocaparvoviruses (DBoVs), DBoV1 and DBoV2, from dromedary faecal samples in Dubai. Among 667 adult dromedaries and 72 dromedary calves, 13.9 % of adult dromedaries and 33.3 % of dromedary calves were positive for DBoV1, while 7.0 % of adult dromedaries and 25.0 % of dromedary calves were positive for DBoV2, as determined by PCR. Sequencing of 21 DBoV1 and 18 DBoV2 genomes and phylogenetic analysis showed that DBoV1 and DBoV2 formed two distinct clusters, with only 32.6-36.3 % amino acid identities between the DBoV1 and DBoV2 strains. Quasispecies were detected in both DBoVs. The amino acid sequences of the NS1 proteins of all the DBoV1 and DBoV2 strains showed <85 % identity to those of all the other bocaparvoviruses, indicating that DBoV1 and DBoV2 are two bocaparvovirus species according to the ICTV criteria. Although the typical genome structure of NS1-NP1-VP1/VP2 was observed in DBoV1 and DBoV2, no phospholipase A2 motif and associated calcium binding site were observed in the predicted VP1 sequences for any of the 18 sequenced DBoV2, and no start codons were found for their VP1. For all 18 DBoV2 genomes, an AT-rich region of variable length and composition was present downstream to NP1. Further studies will be crucial to understand the pathogenic potential of DBoVs in this unique group of animals.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Chow, Franklin W N" sort="Chow, Franklin W N" uniqKey="Chow F" first="Franklin W N" last="Chow">Franklin W N. Chow</name><name sortKey="Joseph, Sunitha" sort="Joseph, Sunitha" uniqKey="Joseph S" first="Sunitha" last="Joseph">Sunitha Joseph</name><name sortKey="Lau, Susanna K P" sort="Lau, Susanna K P" uniqKey="Lau S" first="Susanna K P" last="Lau">Susanna K P. Lau</name><name sortKey="Muhammed, Rubeena" sort="Muhammed, Rubeena" uniqKey="Muhammed R" first="Rubeena" last="Muhammed">Rubeena Muhammed</name><name sortKey="Patteril, Nissy Georgy" sort="Patteril, Nissy Georgy" uniqKey="Patteril N" first="Nissy Georgy" last="Patteril">Nissy Georgy Patteril</name><name sortKey="Tsoi, Hoi Wah" sort="Tsoi, Hoi Wah" uniqKey="Tsoi H" first="Hoi-Wah" last="Tsoi">Hoi-Wah Tsoi</name><name sortKey="Wernery, Ulrich" sort="Wernery, Ulrich" uniqKey="Wernery U" first="Ulrich" last="Wernery">Ulrich Wernery</name><name sortKey="Wong, Emily Y M" sort="Wong, Emily Y M" uniqKey="Wong E" first="Emily Y M" last="Wong">Emily Y M. Wong</name><name sortKey="Woo, Patrick C Y" sort="Woo, Patrick C Y" uniqKey="Woo P" first="Patrick C Y" last="Woo">Patrick C Y. Woo</name><name sortKey="Yeung, Hazel C" sort="Yeung, Hazel C" uniqKey="Yeung H" first="Hazel C" last="Yeung">Hazel C. Yeung</name><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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